Protein Screening Process on our Robotic Platform

Scheme 1:Protein Screening Process

 
The screening process can be devided into  steps:

1. (semi-) rational design

2. mutagenesis protocols

3. transformation of the mutated gene into an expression organism

Steps in the platform

A + B) expression of the enzyme

C) harvesting the cells

D)  extraction of the enzyme of interest

E) quantification of the enzyme activity

F) automated statistical analysis with R

optional reiteration of the steps 1 to 8.